>
FINAL REPORT
INTEGRATION OF MULTIMEDIA AND INTERNET RESOURCES
INTO COURSES IN BIOLOGY AND CHEMISTRY
1. Project Dates:
Project Start Date: 1 June 1995
Project End Date: 30 November 1995
2. Key Contact Person:
Gordon Schrank, MS-228, Biological Sciences, COST
Phone: 255-3047
Fax: 255-4262
Email: SCHRANK@tigger.stcloudstate.edu
3. Other Participants:
Denise McGuire, MS-272, Biological Sciences, COST
Phone: 255-4975
Fax: 255-4262
Email: MCGUIRE40@tigger.stcloudstate.edu
David DeGroote, MS-225, Biological Sciences, COST
Phone: 255-3207
Fax: 255-4262
Email: DEGROOTE@tigger.stcloudstate.edu
Neal Voelz, MS-222, Biological Sciences, COST
Phone: 654-5427
Fax: 255-4262
Email: NVOELZ@tigger.stcloudstate.edu
Mehroo Cooper, MS-364, Chemistry, COST
Phone: 255-2024
Fax: 255-4262
Email: MCOOPER@tigger.stcloudstate.edu
4. Project Summary:
The objective of this project is to provide a
mechanism to integrate the plethora of instructional
aids (macroscopic and microscopic images, figures, data,
graphs, molecular models, etc.) into laboratory and
lecture experiences for students in biology and
chemistry. We currently have pieces of the technology
and Ethernet connections that allow small groups of
students to use some of this type of information. In
biology, two computers were purchased with Title III
funds. An individual or small groups of students can
make use of this technology. We have found two major
problems with our current system. First, the number of
students who can participate in the use of equipment is
limited. Second, we have no way to integrate materials
produced by students or faculty into the system. Third,
we have no way of utilizing any of this technology
during lectures.
This proposal will alleviate these problems by
providing two types of equipment. First, funds are
requested to purchase video interface equipment to
project computer generated images, videos, graphs, text
and other materials. We have two projectors available
for use but need the computers and video converter
system to use the projectors. Second, equipment is
requested for an authoring workstation (equipment and
work area to produce multimedia materials). Both
students and faculty can digitize or record images,
text, graphs and other resource materials. This
information can be used with existing programs such as
HyperCard (MacIntosh computers) and ToolBox (IBM and
IBM-clones) to make multimedia presentations. We find
students have a real interest in using their own
materials to demonstrate principles and themes in
scientific disciplines, especially materials they have
collected themselves in the laboratory. This ensures
ownership and generates pride not only in understanding
material but also assisting others to assimilate
material through learning aids generated by students.
Often this provides a new perspective on how to
introduce and explain a particular topic in that we have
noted that peer-level interactions provide an additional
dimension to understanding in student groups.
SPECIFIC QUESTIONS RELATING TO PROJECT:
1. How did students/faculty assess the experience?
Students respond very favorably to the projection
system. All students in a class are able to see what
appears on the computer screen. It has been very useful
in demonstrating Internet access, "how to" sessions and
to provide a general overview of computer usage and
details of specific computer programs.
The development of multimedia materials is in
progress. Students in particular classes (Biology 366 -
Light Microscopy) have made extensive use of the
equipment. Other students/faculty targeted for this
equipment have not made as much use of the equipment
largely because of the learning curve associated with
the new equipment and software. We have one student
(Patty Wehlan) with extensive computer experience who is
developing tutorials for both faculty and students.
2. What evaluation parameters did you use and why?
To date, where the equipment is in place, students
have been evaluated by oral and computer exams (skills
demonstration test - sample sheet is attached) and
electronic portfolio development. As more classes make
use of the equipment, we will have a larger database for
evaluating outcomes. The integration of this equipment
with other work areas in biology is discussed in the
attached paper An Assessment of New Technologies in Biology Laboratory
Courses and Student Research which was presented in November,
1995, at the Technology in Education conference held in Minot,
North Dakota.
3. Is on-going discussion across disciplines and between
institutions feasible or desirable?
The Minot conference provided many contacts which
Gordon Schrank has maintained. The use of such
equipment and ways to maximize its impact in courses is
being studied. We expect many more developments in this
area and plan to report results at the next conference
in Minot scheduled for November, 1996.
We are working to establish dialog within the
College of Science and Technology. Gordon Schrank is
serving as a facilitator for the Brown Hall computer
laboratory and hopes to integrate some of the multimedia
results into activities held in this room. This
continuation of the project is currently in the planning
stages.
The new equipment and proposed uses has spawned a
grant proposal to the National Science Foundation. This
proposal, submitted in November, 1995, requests a server
system to make teaching materials produced with the Q-7
multimedia equipment available on campus for use in
various computer labs and by students at home via phone
connections.
4. How was learning affected (method, didactic, and resources),
in the long- and short-term.
Since the computer resources were put into place in
early fall, outcomes are for the short-term. The major
impact has been noted in Biol. 366 where the equipment
is used extensively. Student groups use items for
individual laboratory exercises. The projection system
has been used for Biol. 201 (General Biology), Biol. 344
(Microbiology) and Biol. 445 (Medical Microbiology). In
addition, demonstrations have been given in a graduate-
level course for teachers.
We anticipate making many study items available to
students via CD-ROM or through the network. Classes
such as histology, with extensive use of prepared
microscopic slides, have need of tutorials. Tim Schuh
and work study students have just begun a consideration
of integrating slides into computer tutorials. We
expect this project to be completed by the end of next
summer.
5. How were objectives of learning affected (e.g., concept
building, abstraction)?
We report considerable progress in the concepts
relating to building useful electronic presentations.
Students, in general, are most receptive to using both
the software and hardware. We find that they are very
able in terms of expanding the basic uses which they are
asked to learn. For example, students using image
analysis software (software used to measure and label
components of images) found ways of exporting results
into spreadsheets and other programs to produce graphic
results of measurements.
6. What are the challenges and benefits of collaborative
efforts?
Not surprisingly, involving everyone in the
collaborative project is difficult. We are trying to
provide a bridge between faculty users through students
who have been trained to use the equipment. These
students have been instrumental in developing uses of
the programs and greatly enhanced the use by suggesting
possible uses of data and improvements for tutorials.
RECOMMENDATIONS AND FUTURE WORK:
1. Outline an improved design for curriculum of participating
programs or departments.
We have an excellent opportunity to use this
equipment along with the Title III computer room located
in Brown Hall. If the server proposal is funded, all
faculty and student tutorials will be available for
review and use by students. If this proposal is not
funded, we will make more use of the CD-ROM producing
capability of our equipment and make CDs available for
use in the same facility.
We anticipate developing better study guides and
tutorials of biologically active chemical compounds.
This endeavor will involve faculty from both biology and
chemistry. Students in both programs, particularly
biomedical science and the chem-med degree option, will
benefit from this type of work.
2. Comment on areas of learning that lend themselves to cross-
disciplinary endeavors.
Tutorial development involves not only the
knowledge-base departments but also the input of
personnel in the Learning Resources Center and the
College of Education. Effective development requires
tutorials which are clear, usable, challenging and based
on sound pedagogy. Input from professionals on campus
is important in development of these tutorials.
3. Suggest benefits of continued exchange of knowledge between
departments/disciplines.
We hardly need to comment on the knowledge
explosion occurring in all disciplines. Biochemistry,
which can serve to link biology and chemistry, is best
explored through the professional interaction of
biologists and chemists.
4. Address potential for permanent academic exchanges between
the parties.
Integration of biochemical concepts into tutorials
is expected to continue. Presentation of concepts and
the different perspectives from biology and chemistry
are very important. It is clear from student assessment
that the best prepared and most successful graduates in
biology programs have a strong understanding ability to
use chemical concepts.
5. If applicable, suggest disciplines that should have been
included but were not.
Though we have worked with individuals in the
Learning Resources Center, we feel their input would
have been valuable in the early planning. However, we
have made contact with them regarding not only
development of tutorials and related materials, but have
attempted to determine how these materials might be used
in "distant learning" initiatives which are being
developed on our campus.
===========================================================================
===
FINAL ACCOUNTING OF EXPENDITURES AND
JUSTIFICATION:
(10) CD-R 74-Min, 640MB $96.90
(1) Omnipage Pro. 324.00
(2) Powerpoint 196.00
(2) Power Mac AV Card 780.00
(2) Apple Design Keyboard 144 .00
(2) Apple Multiscan 15 Display 742.00
(1) Stylewriter 1200 229.00
(12) Stylewriter ink cartridge 216.00
(4) Memory for Power Pac 1216.00
(2) Apple Design Powered Speakers 274.00
(1) Cable set for MAC SCSI devices 59.00
(2) Maclink Plus 198.00
(1) Etherlink 10BaseT card 160.00
(2) Power Mac 7100 3080.00
(2) Authorware 1740.00
(1) 2150 MB Ext. HD 953.00
(1) Mac Pinnacle CD 1528.00
(1) Apple Color One Scanner 715.00
(1) Proxima 2750 Desktop Projector 6715.00
TOTAL $19365.90
The original budget specified both IBM and
MacIntosh computer systems along with appropriate
software for creating multimedia presentations. Gordon
Schrank attended two courses using multimedia authoring
tools for IBM-compatibles only. This experience, along
with the availability of the Title III computer
laboratory in Brown Hall led participants to consider
MAC only systems. The software selected does have
cross-platform capabilities.
Specific software selected includes OmniPage for
use with the Apple Color Scanner, PowerPoint (cross-
platform presentation software) and AuthorWare (cross-
platform multimedia authoring program). The MacLink
Plus is a filtering tool software package which
facilitates importing of files into different program.
Efforts are being directed at obtaining Adobe Photoshop
to facilitate better handling of graphics in terms of
making uniform sizes. We anticipate purchase of this
program within the next year.
Hardware selected included two Power MacIntosh
computers with audio-visual input cards, keyboards,
speakers, monitors and additional memory (total memory =
24 MB for each computer). These two computers are used
in specific areas.
The first is a multimedia authoring workstation
with a 2.15 GB hard drive, Apple Color One Scanner and
Pinnacle read/write CD-ROM drive. Presentations will be
composed on the large hard drive, files optimized and
then transferred to CD-ROM disks. This computer also
has the Stylewriter 1200 attached for printing.
The second computer is on a cart and has Ethernet
connections and the Proxima 2750 Projector for use in
classrooms and laboratories. Also, graduate students
are composing seminar presentations using PowerPoint.
Miscellaneous items purchased include blank CD-ROM
disks (640 MB capacity) and cables for connecting the
various SCSI devices (scanner, high capacity drive and
read/write CD-ROM). Also, Gordon Schrank has purchased
a digital camera with funds from another grant. This
camera will allow for input of electronic images and
negates the need for film and film processing.
Future grants planned include proposals for
microscopes and digital camera systems for quick capture
of images. We currently have such equipment, but it is
shared and housed throughout the biology department. We
feel such equipment would facilitate input of materials
for multimedia use.
===========================================================================
===
=================================================================
The paper which follows was published in Midcontinent Institute's
Fourth Annual Innovations in Education Conference and Faculty Development
Workshops held November 9-12, 1995, in Minot, North. The Conference was
sponsored by Minot State University. Gordon Schrank also gave an oral
presentation on November 12,1995, in Minot, N.D.
================================================================
=
An assessment of new technologies in biology laboratory courses
and student research
_________________________________________________________________
GORDON SCHRANK, DENISE McGUIRE, JANET WOODARD and DAVID DeGROOTE
St. Cloud State University
Since 1987, we have received four National Science
Foundation (NSF) grants and one U.S. Department of
Education Title III grant for laboratory improvement.
The equipment includes instrumentation for analytical
chromatography, immunology, microscopy and image
analysis and an introductory cell biology laboratory.
More recently we received funding from the Minnesota
State University System (Q-7 Funding Initiative for
increasing scientific and quantitative literacy) for an
authoring workstation which will allow us to integrate
student work from these various areas and make it
available for students reports, lectures and review
through a computer network. This latter workstation
will allow faculty and students to use actual data in
seminars, recitation sections and in lecture or review
sessions. The impact of this technology goes far beyond
the courses for which it was originally requested. It
has helped students with job skills and placement,
preparation for graduate school and, in the various
courses, we have seen a dramatic increase in the quality
and complexity of analysis in student reports. Also, we
have noted an increased sense of ownership by students
using this equipment. As the computer network
technology is put into place, we anticipate a comparable
improvement in student oral presentations.
St. Cloud State University has evolved from a
normal school for teacher preparation to a comprehensive
university with a primary focus on undergraduate
education. The mission of faculty within the Department
of Biological Sciences has paralleled that of the
university with an emphasis on preparation of
undergraduates for jobs, graduate or professional
school. Also, the Department has maintained a graduate
program for students wishing to complete a Master's
degree. For the past decade, efforts have been directed
at providing state-of-the-art experiences in scientific
techniques for both undergraduate and graduate students.
It is obvious that external funding plays a vital role
in helping academic departments purchase scientific
equipment. It is also important to provide ways to
share information and results from the use of this
instrumentation. The narrative which follows discusses
the equipment purchased through external funding and the
most recent efforts to provide a way to electronically
archive and use information from laboratory experiences.
DESCRIPTION OF EQUIPMENT
________________________________________________________________
Chromatography Facility
This area consists of two high performance liquid
chromatographs (Hewlett-Packard HP1090 and Shimadzu SCL-
6A Liquid Chromatograph) and a gas chromatography system
(Hewlett-Packard 5890). In general, chromatography
deals with the separating, identifying and quantifying
organic compounds. In biology, these samples may be
taken from various sources including plant and animal
tissues and fluids as well as products produced by cells
in culture and by microorganisms. The quantifying and
identification of molecules is based largely on
comparison with known standards. These instruments have
three major components which are the injector area,
separation column and detector system. The sample is
introduced through the injector and then components of
the sample interact with materials in the column. The
construction of the column is critical to the separation
of the various molecules. Materials with a high
affinity for materials in the column are retained longer
than materials with low affinity (Moss, 1985). The time
that a compound remains in the column is called the
retention time.
As materials emerge from the column, some type of
detector system is used to demonstrate their presence.
Detectors include those that measure the absorption of
ultraviolet (UV) or visible light, fluorescence activity
of sample compounds, conductivity, amperometric
properties or refractive index (Lindsay, 1987).
In gas-liquid chromatography (GLC), the sample and
a carrier gas are injected and the sample is converted
to a vapor state. The volatilized components emerge
from the column based on their affinity for the liquid-
coated inert support within. In high performance liquid
chromatography (HPLC), the specimen is dissolved in a
solvent which is pumped under high pressure through the
column. Again interactions with components of the
column determine when various compounds will leave the
system. Many different types of columns are available
for analytical purposes. After purchase of the primary
instrument, different columns may be used as need for
accurate separation and identification of columns (Moss,
1985). Computer components control pressure,
temperature and other features of operation and provide
a way to store data and print chromatograms for use in
teaching and scientific presentations.
Both GLC and HPLC have a wide range of applications
in biology including identification of toxic compounds
in environmental samples, identification and quantifying
of materials in tissues such as hormones, analysis and
purification of DNA components such as plasmids from
microorganisms and many other products produced by
living systems.
Currently, this instrumentation is used by six
faculty members for training in seven classes. Four
faculty are using the facility for research and a number
of undergraduate and graduate students have completed
individual work resulting in presentations and published
papers. Examples of uses of the facility include ion
exchange chromatography to separate proteins,
extrachromosomal DNA and volatile organic acids.
Reverse phase chromatography has been used to separate
proteins, alkaloids and naturally occurring plant growth
regulators. Faculty and students have found the
instrumentation to be accurate, reproducible and
efficient.
Fluorescent Microscopy Workstation
Viewing biological specimens with white light can
provide useful information regarding structure and
organization of biological tissues and specimens. Many
biological specimens contain components which react to
specific wavelengths of light. Short wavelengths (near
the blue end of the spectrum) will excite these
components which then give off an emission
(fluorescence) of longer wavelengths (near the green to
red portion of the spectrum). For example, different
types of chlorophyll in plants respond to this type of
stimulation. In addition, many fluorescent dyes can be
used to stain certain tissue components. Some such as
acridine orange, have an affinity for nucleic acids.
Therefore, the portion of a cell which contains
fluorescence is known to have high levels of nucleic
acid. Finally, certain fluorescent dyes can be linked
to antibodies to demonstrate the presence of specific
compounds in tissues.
This type of instrumentation may provide a visual
image of the location of specific molecules in tissues
and other specimens and can complement other studies
such as those with HPLC which demonstrate that the
compound is present is a particular tissue. The
instrumentation includes a Zeiss microscope capable of
both transmitted fluorescence and epifluorescence
microscopy. The microscope is equipped with a silicon
intensified tube (SIT) camera and VHS/SVHS video
recording system. The instrumentation has enhanced
training in a number of areas. These include courses in
light microscopy and photomicrography, immunology,
microbiology and electron microscopy.
The instrumentation has been used extensively in a
number of areas. First, demonstrations for visiting
high school classes include both light and electron
microscopy. Tour groups are given demonstrations of a
fluorescent-tagged antibody preparation (commercial
slide kit for herpes diagnosis) and a fluorochrome stain
for acid-fast bacteria (auramine-O and rhodamine stain).
Second, the instrumentation is used for teacher training
sessions and workshops to enable junior high, middle
school and high school teachers to more effectively
describe this type of instrumentation to their classes.
Third, the instrumentation is an integral part of
teaching laboratories for a number of our courses.
Specific examples in general and medical
microbiology include autofluorescence of fungi,
fluorochrome differential staining and fluorescent
antibody techniques. For our light microscopy course,
the instrumentation is used to demonstrate infected
plant cells, bacteria in root nodules, staining for
lipid inclusions and lysosomal granules and trace metal
detection in granulocytes. For our immunology
laboratory, students used the microscope to view
phagocytosed yeast or bacteria and fluorescent antibody
preparations.
Individual student projects using epifluorescence
microscopy have included demonstration of bacterial
adherence to biomaterials such as catheters and contact
lenses.
Image Analysis Workstation
The primary instrumentation for this workstation
includes the VIDAS image analysis system (Carl Zeiss,
Inc.) and a high resolution black and white camera (Dage
CCD 72).
The human brain is very well suited for responding
to visual stimuli. We can readily distinguish fine
detail in images presented for viewing. However, most
of us have some difficulty in routinely measuring
components in images that we see. Even when this is
possible, it become tedious to repeat the procedure
hundreds of time (Russ, 1990). However, in biology, we
often need this type of quantitative information.
Computers can repeatedly make measurements, calculate
and tabulate results. Image analysis involves an
interaction between an operator who, through a series of
steps, tells the computer what to measure. Once this is
established, the computer can make these measurements on
any number of samples. This technique is useful with
microscopic images as well as stored images of
structures which are visible to the unaided eye.
Data analysis in biology is based largely on the
collection of images. Examples include images produced
from molecular analysis of vital molecules in gels and
from cells and those seen in various types of
microscopes. The department has a large inventory of
image gathering devices. Our instrumentation was
deficient in one major area, i.e., image analysis and
measurement. The acquisition of an image analysis
system has allowed us to train students in collection,
storage and interpretation of data acquired from
morphological studies of cells and their structural
components. Additionally, students have been able to
quantify results obtained from molecular biology
experiments such as gel electrophoresis of cell lysates.
Finally, with the macro lens system, students have
viewed and quantified growth changes in small aquatic
plants. The instrumentation has been successfully used
with an NSF sponsored Young Scholars program as well as
regular undergraduate and graduate classes at our
institution. We feel the training has assisted with
increasing both computer literacy and experience with
state-of-the-art image analysis. Because of specialized
features such as automatic macro writing, the system is
relatively user friendly and allows students to
visualize computer processing mechanisms without first
acquiring programming skills. The flexibility of the
system's software has allowed students to understand
that quantifying may be approached from different
perspectives in terms of preprocessing images. Since
the instrumentation can rapidly do the analysis,
students feel free to experiment with data evaluation.
Immunology and Cell Culture Facility
Both immunology and cell culture represent areas of
biology that provide essential tools and techniques for
the study of biological questions. Immunology is the
study of the immune system and how organisms protect
themselves against foreign substances and abnormal cells
within their own system. A very important part of the
immune response is the production of antibodies. These
antibodies may be used as reagents in many ways
including demonstrating the presence of specific
compounds in tissues. Thus, like HPLC and specialized
forms of microscopy, techniques using antibodies can
help us to understand where specific compounds occur in
organisms and how they are produced.
Cell culture is the growth and multiplication of
cells in an in vitro environment. Growth of individual
cells can often answer questions about specific products
synthesized by an organism. This type of culture also
allows testing of the effect of substances on the growth
and reproduction of cells.
A number of items were purchased for this facility.
Of particular note are a special incubator for cell
growth (carbon dioxide incubator) and equipment for
following specific types of immunological reactions.
The latter includes a Dynatech ELISA plate washer and
microplate reader for enzyme immunoassays.
Enzyme immunoassays are very sensitive tests that
allow for detection of minute quantities of materials.
Nakamura and Tucker (1984) describe this assay as being
sensitive due to the amplification effect of enzymes,
relatively inexpensive with regard to reagents which
have a reasonable shelf-life, safe with no radiation
hazards and an assay with may be easily automated.
These advantages are useful for student training in that
the best alternative in terms of sensitivity involves
the use of antibodies labelled with radioactive
compounds. Use of these reagents significantly increase
costs and the handling and disposal of radiolabelled
compounds poses special problems.
The facility serves as a resource for classes
involving immunological and cell culture techniques. A
wide variety of undergraduate student projects have made
use of the facility. Examples include the effect of
bacterial toxins on cells, the growth and development of
cells into organized units, establishment of plant cell
culture and the effect of trace elements on plant cell
culture.
Title III Cell Biology Laboratory
The primary goal of this facility is to provide an
introduction to laboratory techniques and skills used in
all of the biological sciences and an introduction to
data analysis. This grant represented an enhancement to
the existing curriculum. The equipment purchased
involved many standard laboratory items such as
balances, power supplies and related materials for
electrophoretic separation of compounds found in fluids
such as blood and computer hardware and software for
data analysis and presentation.
Multimedia Facility
As mentioned, a major goal of our grant activities
was to develop a way to share information and
techniques. All of the work areas described help to
detect and at least partially quantify molecular
components of living systems. Output from these areas
can be visual images, graphs, numeric data and standard
and enhanced micrographs. The multimedia facility
provides a way for students and faculty to bring
together data and images to present the information.
Equipment purchased for this facility include two
Power Macintosh 7100 AV computers, a color scanner,
read/write compact disk drive, multimedia software
(Authorware 2.2) and a high resolution projector for
displaying computer images and slides shows. These
items, along with existing color cameras for microscopes
allow for the input of images and data from laboratory
exercises, undergraduate, graduate and faculty research.
SUMMARY
1. External funding has allowed for the development of
several facilities or workstations which have enhanced
our curriculum and provided research opportunities for
faculty and for undergraduate and graduate students.
2. Acquiring external funding for equipment allows
academic departments to invest their regular funding in
upgrading existing equipment and providing supplies for
items acquired through grant programs.
3. Providing state-of-the-art equipment for student
training and research consistently enhances student
performance in terms of finding jobs and applying for
postgraduate training.
4. We consistently find that this equipment provides
students with tools for investigation beyond the
original intent of the laboratory exercises. All of the
computer based equipment allows students to acquire and
analyze data and develop skills in presenting their
findings. This enhances their feelings of ownership and
excitement about the work. The addition of equipment to
produce slide shows and various multimedia presentations
enhances even more the enjoyment of collecting,
analyzing and presenting data.
5. New equipment and facilities may be a recruiting
tool for incoming undergraduates and plays a vital role
in ongoing teacher enhancement programs.
Notes
The authors wish to acknowledge funding for the projects described in
this article. These include NSF Project 8750520 (Analytical Chromatography
Facility), NSF Project 8852090 (Immunology/Cell Culture Facility), NSF
Project 8950551 (Fluorescent Microscopy Workstation), NSF Project 9051304
(Image Analysis Workstation), Q-7 Scientific and Quantitative Literacy
Project (Multimedia Facility), and Title III Funding to State Cloud State
University, Undergraduate Investigative Studies in the College of Science
and Technology (Cell Biology Laboratory Development).
Correspondence regarding this article should be addressed to Gordon
Schrank, Department of Biological Sciences, St. Cloud State University, MS-
228, 720 4th Ave. S., St. Cloud, MN 56301-4498.
click here for part two
Return Q7RWC-outreach